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KMID : 0525720130180040250
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Journal of Chitin and Chitosan
2013 Volume.18 No. 4 p.250 ~ p.255
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Effects of Proton Beam Irradiation on Chitosan Oligosaccharide-induced Inhibition of Metastatic Factors in Breast Cancer Cells
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Lee Do-Hyung
Lee Kyu-Shik
Nam Kyung-Soo
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Abstract
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We investigated the effect of proton beam irradiation on chitosan oligosaccharide (COS-L, MW ≤ 1,000 Da)-mediated inhibition of metastatic potential in MCF-7 human breast cancer cells. 8 Gy and 16 Gy of proton beam irradiation enhanced COS-Lmediated down-regulation of cyclooxygenase-2 (COX-2) induced by phorbol-12-myristate-13acetate (TPA). Also, matrix metalloproteinase- 9 (MMP-9) expression was synergically suppressed by chitosan oligosaccharide and proton beam irradiation. Furthermore, 8 Gy and 16 Gy of proton beam enhanced down-regulation of chitosan oligosaccharide-dependent urokinase plasminogen activator (uPA) expression. However, that of uPA receptor (uPAR) was not affected by proton beam irradiation. These results suggests that proton beam may enhance chitosan oligosaccharide-mediated inhibition of metastatic potential in MCF-7 human breast cancer cells via further decrease of COX-2, MMP-9 and uPA expressions. Therefore, these results suggest that combinational treatment of chitosan oligosaccharide and proton beam irradiation may use effective strategy for preventing breast cancer development, growth and metastasis.
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KEYWORD
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Proton beam, Chitosan oligosaccharide, Breast cancer, Metastasis
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